This Research Deconstruction – Introductory Biology module contains content designed to be easily added to your biology course: a research seminar video and a series of tutorial videos, as well as sample syllabi as examples for how you might integrate the material with your syllabus.  The research seminar provides the source material for the deconstruction.  The tutorial videos are intended to support the deconstruction by introducing students to fundamental concepts or techniques used in the research.  They can be assigned for pre-class viewing and then reviewed in class during discussion of experiments or other content from the seminar.

These video resources are available at Canvas Commons.  An overview of each video is provided below.  You can read the overview to decide whether or not the video is relevant to your course.

This material is openly licensed via CC BY-NC-ND 4.0.

Research seminar – Stephanie Correa (UCLA)

This seminar, by Dr. Stephanie Correa of UCLA, describes the identification and molecular and genetic analysis of a population of estrogen-responsive neurons in the hypothalamus that are responsible for sex-specific differences in body temperature.  The experiments and underlying science connect to several topics from introductory biology, including homeostasis, cell signaling, gene expression and proteins, all of which are relevant to the standard syllabus from introductory biology.

Tips and Pointers on Integrating Content with your Syllabus

Research deconstruction is flexible, and there are multiple ways that you can incorporate it into your class. However, the following pointers are worth keeping in mind:

  • As you watch the seminar for the first time, try to find points of alignment between concepts or experimental techniques from the seminar and concepts covered in your regular course syllabus. These will provide opportunities for you to use part of the seminar to show how the concept(s) you are teaching in your class relate to the research presented by the speaker. Two sample syllabi are presented below.
  • Most faculty have their class watch the seminar early in the semester, so they can deconstruct it gradually over the course of the semester.
  • Keep in mind that your students will understand very little of the seminar when they first see it. You will need to reassure them that this is expected, and that they will understand much more of it by the end of the semester.
  • Because this is more advanced material than students usually encounter in an introductory course, we recommend that any assessments related to topics from the seminar be low stakes and graded on effort rather than accuracy.
  • One low stakes assignment you might consider is a reflection piece that you can assign immediately after students view the seminar, and again at the end of the course, after the material has been deconstructed.  This helps students see how much they’ve learned over the course of the semester. A sample reflection assignment is available below.
  • To help facilitate discussing advanced techniques such as immunostaining, we have produced a series of video tutorials. You can assign a tutorial for students to watch before class, and then you can review the content in class while connecting it to material from your syllabus and/or experiments from the seminar.
  • It is not necessary to use all of the video tutorials for this module. You can use as many or as few as you wish.
  • Finally, while you want to maintain the thread of the story in deconstructing the research seminar in your course, you do not need to cover the entirety of the seminar. It is perfectly fine to leave out some parts, due to timing or the complexity of the material. It is more important that students understand the parts that are covered.

Pre-class video tutorials

Scientific Method

Part 1: Introduces and explains the scientific method. (11:35)

Part 2: Describes considerations of testing alternative hypotheses, and explains the value of experimental controls. (14:40)

Cell Signaling

Part 1: Introduces and explains a signal transduction pathway using a generic example. (14:38)

Part 2: Describes phosphorylation as one example of how molecular targets can be switched into active or inactive states. Also mentions how the complexity of a signal transduction pathway is advantageous for a cell. (15:08)

Part 3: Estrogen signaling is used as a specific example of a simple signal transduction pathway. (5:17)

Immunostaining

Part 1: Introduces the concept of immunostaining and explains the importance of tissue fixation. (8:33)

Part 2: Introduces the idea of the primary antibody and describes the basic characteristics of antibodies in general. (6:16)

Part 3: Describes the use of the primary antibody and introduces the concept of the secondary antibody. (5:53)

Part 4: Describes the use of the secondary antibody to produce a detectable visible signal. (6:12)

Gene Expression and Manipulation

Part 1: Introduces basic concept of gene expression, focusing on the role of promoter sequences. (7:37)

Part 2: Establishes that promoter regions can be uncoupled from their native gene sequences. (2:40)

Part 3: Explains how promoter and gene sequences can be artificially “mixed and matched” in experimentally useful ways, and uses the Cre-loxP system to provide one specific example. (3:14)

Part 4: Provides practical considerations when manipulating gene expression, focusing on the concept of modularity. (20:21)

Data Analysis

Part 1: Uses a generic example to explain how all data sets are inherently flawed and explains the concept of data sampling. (24:34)

Part 2: Describes the difference between descriptive and inferential statistics. (12:52)

Part 3: Explains important considerations when designing research questions, applying inferential statistics and generating valid conclusions. (8:32)

In Situ Hybridization

Part 1: Introduces the importance of gene expression profiles and summarizes the central dogma. (7:28)

Part 2: Describes the generation and use of labeled sense and antisense probes. (9:01)

Part 3: Explains the key steps in in situ hybridization using chemically labeled probes as an example. (15:54)

Confocal Microscopy

Part 1: Introduces the principle of confocal microscopy. (11:29)

Part 2: Describes how images can be captured in multiple focal planes to produce three-dimensional views of the fluorescent signal. (3:13)

Part 3: Explains the concept of colocalization when more than one fluorescent signal is being detected in the same sample. (10:01)

Biology (STEM majors) - sample syllabus #1
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Biology (STEM majors) - sample syllabus #2
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Course Schedule

January 2025
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